Part:BBa_M45424

Alkane hydroxylase (Pseudomonas oleovorans)

This protein belongs to the oxidoreductase family of enzymes. In the presence of rubredoxin, alkane hydroxylase catalyzes the aerobic breakdown of an alkane into a primary alcohol and water. Alkane hydroxylase, together with rubredoxin, has also shown catalytic function in the break down of polyethylene plastics and other hydrocarbons [1,2].

Sources: [1]van Beilen, J.B. & Funhoff, E.G. Alkane hydroxylases involved in microbial alkane degradation. Applied Microbiology and Biotechnology (2007) 74: 13. https://doi.org/10.1007/s00253-006-0748-0

[2]Yoon MG, Jeon HJ, Kim MN. Biodegradation of Polyethylene by a Soil Bacterium and AlkB Cloned Recombinant Cell. Journal of Bioremediation & Biodegradation (2012) 3:145. doi: 10.4172/2155-6199.1000145

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  Illegal BamHI site found at 1068
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1097